Advanced Microscopy in Mycology by Tanya E. S. Dahms, Kirk J. Czymmek

By Tanya E. S. Dahms, Kirk J. Czymmek

The goal of this quantity is to explain the most recent advances in microscopic equipment, together with built-in strategies, as utilized to mycology. every one bankruptcy will offer a quick evaluate of a specific microscopic approach with linked benefits and obstacles, the examine questions that may be effectively addressed utilizing those microscopic tools, the way it has been effectively utilized to handle mycological learn questions, together with helping and complimentary innovations, and which destiny questions may be addressed.

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Mol Microbiol 71(1):172–184. (Available from PM: 19007409) Garzia A, Etxebeste O, Herrero-Garcia E, Ugalde U, Espeso EA (2010) The concerted action of bZip and cMyb transcription factors FlbB and FlbD induces brlA expression and asexual development in Aspergillus nidulans. Mol Microbiol 75(5):1314–1324. (Available from PM: 20132447) Gibbs PA, Seviour RJ, Schmid F (2000) Growth of filamentous fungi in submerged culture: problems and possible solutions. Crit Rev Biotechnol 20(1):17–48. (Available from PM: 10770226) Giraldo MC, Dagdas YF, Gupta YK, Mentlak TA, Yi M, Martinez-Rocha AL, Saitoh H, Terauchi R, Talbot NJ, Valent B (2013) Two distinct secretion systems facilitate tissue invasion by the rice blast fungus Magnaporthe oryzae.

FRET is a method that can be used to track dynamic interactions based on energy transfer between two chromophores. 2a) through nonradiative dipole–dipole coupling. The efficiency of this energy transfer is inversely proportional to the sixth power of the distance between the donor and the acceptor, making FRET extremely sensitive to small distances (Tsien 1998). Measurements of FRET efficiency can be used to determine if two fluorophores are within a certain distance of each other, finding wide application in biology and chemistry (Sekar and Periasamy 2003).

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