Animal Virology by David Baltimore (Eds.)

By David Baltimore (Eds.)

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Acad. Sei. USA 69, 24042409 (4) Laub, 0. and Aloni,Y. (1975) Journal of Virol. 16, 11711183 (5) Lindstrom, D. and Dulbecco, R. (1972) Proc. Natl. Acad. Sei.

2 m l of the polyribosomes were incubated with the anti-p30 and S. aureus and all of the RNA was r e l e a s e d from the b a c t e r i a by t r e a t m e n t with 0. 5% SDS p r i o r to r e m o v a l . P r o c e s s i n g was the same as for panel a), c) 1 m l of the polyribosomes was incubated with anti-p30 and b a c t e r i a , and the immune precipitated m a t e r i a l was washed and purified. P r o c e s s i n g was the same as panel a), except that the entire sucrose gradient fractions were concentrated for a n a l y s i s , d) The data from panels b) and c) were normalized to the same peak height of 3 0-3 5S v i r u s specific RNA, and the figures were s u p e r i m p o s e d .

The r e s u l t s are shown in F i g . 4. In these e x ­ p e r i m e n t s , the amount of RNA analyzed from each sucrose gradient was adjusted so that appropriately m e a s u r a b l e amounts of hybridization r e s u l t e d . The p e r c e n t a g e s of cDNA hybridized w e r e converted to relative v i r u s - s p e c i f i c RNA concentration (4), which is plotted. As shown in panel a), v i r u s - s p e c i f i c mRNA in the purified polyribosomes con­ sists of genome length 30-3 5S RNA and in addition v i r u s specific mRNA of s m a l l e r sedimentation value (20-24S in our e x p e r i m e n t s ) , in confirmation of r e s u l t s published by others (10,14).

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