By David Baltimore (Eds.)
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Additional info for Animal Virology
Acad. Sei. USA 69, 24042409 (4) Laub, 0. and Aloni,Y. (1975) Journal of Virol. 16, 11711183 (5) Lindstrom, D. and Dulbecco, R. (1972) Proc. Natl. Acad. Sei.
2 m l of the polyribosomes were incubated with the anti-p30 and S. aureus and all of the RNA was r e l e a s e d from the b a c t e r i a by t r e a t m e n t with 0. 5% SDS p r i o r to r e m o v a l . P r o c e s s i n g was the same as for panel a), c) 1 m l of the polyribosomes was incubated with anti-p30 and b a c t e r i a , and the immune precipitated m a t e r i a l was washed and purified. P r o c e s s i n g was the same as panel a), except that the entire sucrose gradient fractions were concentrated for a n a l y s i s , d) The data from panels b) and c) were normalized to the same peak height of 3 0-3 5S v i r u s specific RNA, and the figures were s u p e r i m p o s e d .
The r e s u l t s are shown in F i g . 4. In these e x p e r i m e n t s , the amount of RNA analyzed from each sucrose gradient was adjusted so that appropriately m e a s u r a b l e amounts of hybridization r e s u l t e d . The p e r c e n t a g e s of cDNA hybridized w e r e converted to relative v i r u s - s p e c i f i c RNA concentration (4), which is plotted. As shown in panel a), v i r u s - s p e c i f i c mRNA in the purified polyribosomes con sists of genome length 30-3 5S RNA and in addition v i r u s specific mRNA of s m a l l e r sedimentation value (20-24S in our e x p e r i m e n t s ) , in confirmation of r e s u l t s published by others (10,14).