Blood Cells, Rheology, and Aging by D. Danon, A. Knyszynski, L. Hayflick (auth.), Professor Dr.

By D. Danon, A. Knyszynski, L. Hayflick (auth.), Professor Dr. med. Dieter Platt (eds.)

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The Effect of Host and Cell Age on the Rat Erythrocyte 49 Weksler: The reason I bring up this question is because many, many rats - I don't know about your type of rat - develop renal disease. And the question is: are we sure that we are not studying the pathology of red cells? Danon: You are dealing with cells that live for a shorter time in the circulation. D. Gershon: Yes. Danon: So, your old cells of fraction 4 are much younger? D. Gershon: They are younger on average, yes. Danon: They are younger.

Glycoproteins bind proportionally less SDS than standard proteins and as a result they have a slower mobility than polypeptides of comparable size. G/ycophorin, the major sialoglycoprotein, contains glycophorin A (PAS-1 and PAS-2), glycophorin B (PAS-3) (Marchesi et al. 1976), and band 3 protein - com- D. Platt and W. S. SDS - polyacrylamide gel electrophoretogram of membrane proteins from young (1,3, 5, 7,9) and old (2, 4, 6, 8, 10) human red cells. Proteins are stained with PAGE-blue 83 ponent III of Fairbanks (1971).

41. 42. 43. 44. the female in aging and the menopause. Results from three population studies. Clin Chim Acta 79:299 Takeuchi N, Matsumoto A, Katayama Y, Arao M, Koga M, Nakao H, Uchida K (1983) Changes with aging in serum lipoproteins and apolipoprotein C subclasses. Arch Gerontol Geriatr 2:41 Vance DE, Sweeley CC (1967) Quantitative determination of the neutral glycosyl ceramides in human blood. J Lipid Res 8:621 Verkleij AJ, Zwaal RFA, Roelofson B et al. (1973) The asymmetric distribution of phospholipids in the human red cell membrane.

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