By Kurt Pfannkuche
This quantity information protocols on detection and research of fusion occasions in a number of cellphone varieties in vitro and in vivo. extra, protocols goal to facilitate the long run hugely suitable means of engineering cells for particular reasons, akin to new release of transgenic embryos and improvement of hybrid cells, on the way to take on particular projects in telephone biology and drugs. Written for the Methods in Molecular Biology sequence, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, simply reproducible laboratory protocols, and pointers on troubleshooting and warding off recognized pitfalls.
Practical and authoritative, Cell Fusion: Overviews and techniques, moment Edition serves as an awesome reference on phone fusion and laboratory protocols.
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Additional resources for Cell Fusion: Overviews and Methods
PLoS One 4:e6530 Johansson CB et al (2008) Extensive fusion of haematopoietic cells with Purkinje neurons in response to chronic inflammation. Nat Cell Biol 10:575 Nygren JM et al (2008) Myeloid and lymphoid contribution to non-haematopoietic lineages through irradiation-induced heterotypic cell fusion. Nat Cell Biol 10:584 Alvarez-Dolado M et al (2003) Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Nature 425:968 Camargo FD, Finegold M, Goodell MA (2004) Hematopoietic myelomonocytic cells are the major source of hepatocyte fusion partners.
However, it had also been shown that the nature of CSC is variable in different cancer types and that CSCs are not necessarily more resistant or slower proliferating than the rest of the tumor cell population [130–133]. Thus, one challenge to overcome in cancer treatment strategies is defining the characterization of these cell subpopulations in the tumor mass. Several studies have shown that cell fusion occurs in solid cancers in vivo between tumor cells and tumor cells or tumor cells and other cells of the tumor environment [40, 41, 86, 90, 134].
27. 28. 29. with different organ or tissue specificity]. Vestn Akad Med Nauk SSSR 26:75 Chakraborty AK, de Freitas Sousa J, Espreafico EM, Pawelek JM (2001) Human monocyte x mouse melanoma fusion hybrids express human gene. Gene 275:103 Rachkovsky M et al (1998) Melanoma x macrophage hybrids with enhanced metastatic potential. Clin Exp Metastasis 16:299 Goldenberg DM (2012) Horizontal transmission of malignancy by cell-cell fusion. Expert Opin Biol Ther 12(Suppl 1):S133 Mi S et al (2000) Syncytin is a captive retroviral envelope protein involved in human placental morphogenesis.