Deutsche Pharmakologische Gesellschaft by (auth.)

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There was a dose depf~dent decrease in the Cd -transfer starting at 10 mg Cd /1 coincidently with a reduction in+the Cd-uptake,which was influenced already ~t 3 mg Cd /1. This influence, however,was greatly reduced, if Zn was withdrawn 18 hours before the experiments were started. Pretreatment with 10 and 100 mg Cd+ /1 lead to a 3 and 11 fold increase of the mucosal Metallothionein (MT) content. An even higher (24 fold) MT-~ontent was detected in rats pretreated with 100 mg zn+ /1. Withdrawal of zn++ 18 hours before the experiments attenuated the MT-content considerably (only 4 fold increase).

0000015% of the total protein content of the pregnant animal. Supported by erants of the DFG to Sfb 29. lnst. f. Dross If the commonly used substrate concentration in acetylcholinesterase(AcChE)-estimations is decreased1 the percentual amount of acetylcholine (AcCh) hydrolysed increases, although absolute activity declines. With respect to the physiological role of AcChE in neurotrans~ mission one has to assume that AcChE works invivo with first-order kinetics. Since the halflife-times or the logarithmic decrements(k) vary linearly with enzyme cincentration, it is possible to estimate half-life-times of tissues from its homogenates.

05 ug Pbac/g). At the same time an organ-specific sensitivity of DAla-D to the duration of the lead effect could be demonstrated. As a measure of this sensitivity we selected the quotients of the lead concentrations which after 10 and 60 minutes' incubation respectively induced a 50-% inhibition of D-Ala-Dactivity (ED-50 (60 min)/ED-50 (10 min)). 33 for the cerebral enzyme. After a single intravenous injection of 100 ug Pbac/kg body weight there was an organ-specific inhibition of the enzyme. Whereas the maximum inhibition of the erythrocytic D-Ala-Dactivity was reached after just 6 hours (57 % of the control activity), inhibition of the enzyme activity in the remaining organs was delayed and did not reach a maximum until 12 hours had elapsed.

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